Organically managed soils show high immunity against a wide range of phytopathogens and can suppress plant disease development. Such immune soils are called general disease-suppressive soils. However, the underlying mechanisms and factors responsible for this phenomenon remain unknown due to limited studies on general disease suppression and heterogeneity of soil. General disease suppression holds promise in achieving sustained agricultural production as it can suppress a wide range of soil-borne pathogens in an eco-friendly manner. Hence, understanding the general disease suppression mechanisms can aid its large-scale application. Therefore, the present study was designed to establish associations between soil biological activity, physicochemical parameters, and disease suppression. Rhizospheric soil samples were collected from comparable organically managed and conventional farming fields in the North-Western Himalayan region of India. Soil samples were screened for in vitro pathogen suppression efficacy against nine fungal and two bacterial phytopathogens. Soil biocontrol enzymatic activities such as chitinase, cellulase, protease, lipase, and siderophore production were determined. Molecular markers for disease suppression were quantified using qPCR, and plant-available nutrients, pH, EC, and organic carbon were determined. The organically managed soils showed higher suppression against all phytopathogens, high biocontrol enzymatic activities, and plant-available macronutrients compared to the conventionally managed soils. Available micronutrients such as Fe, Cu, and Mn were negatively correlated with pathogen suppression. The current study outlines the role of biotic and abiotic factors in general disease suppression in Himalayan soils, highlighting the importance of adapting organic farming to improve soil health and resilience against phytopathogenic attacks.