Probiotics consist of live microorganisms that when consumed in adequate amounts, confer a health benefit to the host. The efficacy of probiotics is largely determined by their ability to survive the transit through the gastrointestinal tract (GIT), including the acidic environment in the stomach, and to adhere and colonize the intestinal mucosa. The low pH levels can have deleterious effects on probiotic microbes leading to protein misfolding and changes in RNA secondary structure formation, which can compromise their ability to survive and colonize the gut. Metabolites secreted by other microorganisms residing the gut can impact the survivability and activities of probiotic bacteria once they reach the colon. This has prompted interest in exploring whether incorporating metabolites from other microorganisms into probiotic products can enhance probiotic viability. For example, spent medium from Saccharomyces cerevisiae strains have been shown to aid the growth of lactic acid bacteria in fermented food products. However, the underlying mechanisms of these bacterial-fungal interactions and their impact on the successful transit of probiotics through the stomach are not well understood. In this study, we aim to investigate the impact of low pH levels, mimicking stomach conditions, on the viability of three Lacticaseibacillus rhamnosus strains. Further, we examine whether incorporating metabolites from Saccharomyces cerevisiae cenpk, derived from cell-free yeast culture supernatant, can improve the resistance of these strains to low pH levels. We utilise a flow cytometry-based approach using dual staining techniques to assess the viability of three known L. rhamnosus spp. under these conditions.