Fungal opportunists cause more than a billion human infections every year, resulting in more than 1.6 million deaths annually and the increase of temperatures is nowadays reported as key-factor for the spreading of multiple potentially infective species. Soils, in particular, have been recognized as one of the major reservoirs of potential fungal opportunists; however, a global ecological focus on diversity and biogeographic patterns of human opportunists in association with environmental and climatic factors across natural biomes is still lacking. To address this knowledge gap, we analyzed a dataset of ITS fungal PacBio sequencing from 3,200 soil samples across the globe from 108 countries spanning all climatic zones, including arid regions. We aimed to (i) explore the global biogeographical patterns of potential fungal opportunists in soil; (ii) elucidate the contribution of climatic and environmental variables to predict the composition and distribution patterns; and (iii) map the current and future distribution of most prevalent fungal opportunistic species in global soils. We found that the abundance of these guilds might increase under higher temperature and solar irradiance, suggesting a greater spread toward temperate-tropical biomes, outlining an expansion of fungal human opportunists distribution due to temperature and aridity variations driven by climate change.